The transgene contains BAC RP23-159N6, in which the zinc finger domain of Prdm9 (coordinates: Chromosome 17, 15680882-15682252, NCBI m37 mouse genome assembly) has been replaced with the zinc finger domain from the Prdm9 allele of strain B10.A(R209)(Prdm9wm7). Line 2 was generated. (J:180577)