一段3kb的基因组区域,包括1号外显子,通过同源重组被pgk-neo cassette替换了。RT-PCR和 Western blot的结果证实了纯合小鼠睾丸中没有表达(来源:J:180915)
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A 3 kb genomic region including exon 1 was replaced with a pgk-neo cassette via homologous recombination. RT-PCR and western blot analysis confirmed the absence of expression in testes from homozygous mice. (J:180915)