FLAG-tagged chicken EphA4 extracellular domain (ECD) fused to the glycosylphosphatidylinositol (GPI) anchor signal of Efna5 was placed under the control of a chicken beta actin/CMV (CAG) promoter behind a floxed stop cassette. The Epha4ECD-Efna5GPI cDNA is followed by an IRES signal driving expression of monomeric membrane-associated cherry fluorescent protein (mCherry). (J:180801)