这个转基因载体包含人RAMP1的cDNA,由CX1启动子调控,CX1启动子是鸡β-actin启动子和CMV增强子的融合产物。载体中插入了RAMP1 cDNA,之后跟着eGFP基因,它被两个loxP位点夹在中间(位于RAMP1上游),并带有β-actin内含子和牛β-珠蛋白的polyadenylation信号。CX1能驱动eGFP的普遍表达,但不表达RAMP1,因为eGFP基因序列中包含终止密码子和polyadenylation信号。只有通过Cre介导的loxP位点上的GFP-stop序列去除后,RAMP1才会开始表达。(来源:J:120077)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Gene Expression
Related Disease
Reference
C57BL/6J x SJL/J
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插入
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1
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6

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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