Del(1Sdccag8-Akt3)CA1C2Ove

A Sleeping Beauty (SB) transposon transgenic approach was used to generate these mice. The pT2-BART3 transposon vector used here has (from 5' to 3') an inverted repeat/direct repeat sequence (IR/DR; the SB transposon recognition site [280 bp]), an AD2 splice acceptor and polyA sequence, the tyrosinase minigene TyBS, a loxP site, a polyA sequence, a Hox9c splice acceptor, and right IR/DR (280 bp). The IR/DR sequences are outward-facing. The 4.1 kb tyrosinase minigene (TyBS) contains the tyrosinase upstream regulatory sequences (2.1 kbp from the BALB/c tyrosinase promoter and the first 65 bp of exon 1), followed by the C57BL/6-derived Tyrs-J cDNA sequence (1.9 kbp including cysteine at amino acid 103, glycine at amino acid 346, and the stop codon with polyA signal). Mice were bred to mice with widespread expression of the SB transposase (CAGGS-SB10 transgenic mice on an FVB/N genetic background). The resulting double transgenic male offspring ("seed males") have the ability to mobilize transposons in their germline; and mobilization of the outward-facing, IR/DR site-flanked transposon results in transposon integration at new sites in the genome (as well as genomic rearrangements including deletions, inversions and translocations). In line OVE2161B.CA1C2, the gene trap cassette inserts between exons 12-13 (178,833,225 bp; NCB137/mm9; L:SV40:178,833,225(+)) and between exons 1 and 2 of the Akt3 gene resulting in a deletion of a 200-250 bp fragment that encompasses exons 13-18 of Sdccag8 and exons 2-13 of Akt3. The absence of transcript expression in both genes was confirmed. (J:180503, J:258011)