外显子1和部分内含子1通过同源重组被替换了反向新插入的neo cassette。 Western blot结果显示,来自纯合小鼠的胚胎成纤维细胞中没有蛋白质表达。(来源:J:178680)
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Exon 1 and part of intron 1 were replaced with an inverted neo cassette via homologous recombination. Western blot analysis confirmed the absence of protein expression in embryonic fibroblasts from homozygous mice. (J:178680)