Pparg^{tm1.1(tTA)Jmgr}

A targeting construct was electroporated into ES cells and by homologous recombination, a tTA-Frt-neo-Frt (tTA = tetracycline transactivator) sequence was inserted into the second coding exon of Pparg, creating an in-frame fusion between the amino terminus of Pparg and tTA. Correctly targeted ES cells were used to generate mice. The neo resistance cassette was removed from the germline by crossing Pparg mutants with FLPe-expressing mice. (J:178521, J:212531)