Tg(Vsx2-cre)2690Chow

Cre recombinase cDNA was cloned downstream of a 5.3kb upstream region of the Vsx2 gene. A 1.9 kb region containing a 164 bp bipolar enhancer was cloned 5' of the 5.3 kb region. An SV40 polyadenylation sequence was inserted downstream of cre. The 1.9 kb region has been identified as a putative retinal enhancer (PRE) and lies within a conserved non-coding region that is within the breakpoint of the Vsx2 (ocular retardation 2J) mutation. 6 founder lines carried the transgene, with 4 (2690, 2695, 2700, and 2717) exhibiting cre expression detected by immunolabeling. (J:178063)