Tg(CD4-Tcra/EGFP,Tcrb)#Mfk

The original OT-I transgenic constructs were obtained from F. Carbone (University of Melbourne, Melbourne, Victoria, Australia). The OT-Ialpha chain was tagged on the C terminus with a 20-aa flexible linker and a single enhanced GFP using standard molecular cloning techniques and was expressed under the CD4 enhancer and promoter without the silencer. The OT-Ibeta chain was expressed under its endogenous promoter. (J:177978)