从2号到4号外显子被替换成一个带有floxed neo cassette的元件。随后通过Cre介导的重组移除了neo cassette。蛋白表达的缺失通过在胸腺细胞中进行 Western blot 分析得到了确认(来源:J:176461)
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Exons 2 through 4 were replaced with a floxed neo cassette. Cre-mediated recombination removed the neo cassette. The absence of protein expression was confirmed by western blot analysis on thymocytes. (J:176461)