Exon 2被替换成一个带有floxed neo cassette的元件。通过在E12.5胚胎提取物中进行 Western blot 分析,确认了蛋白表达的缺失。(来源:J:177092)
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Exon 2 was replaced with a floxed neo cassette. The absence of protein expression was confirmed by western blot analysis on E12.5 embryo extracts. (J:177092)