4号外显子、5号外显子以及大部分6号外显子被lacZ基因和neo cassette替换了。通过在小肠、肺和脾脏的提取物中进行 Western blot 分析,确认了没有蛋白质表达(来源:J:176866)
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Exons 4 and 5 and most of exon 6 were replaced with a lacZ gene and neo cassette. The absence of protein expression was confirmed by western blot analysis on intestine, lung, and spleen extracts. (J:176866)