Ap2b1^{Tg(Tyr)427Ove}

The 4.1 kb tyrosinase minigene (TYBS) transgene with the 2.1 kb BALB/c mouse Tyr promoter region and 2.0 kb Tyr cDNA sequence (including the stop codon polyA sequences) inserted into chromosome 11 in reverse-orientation with the left arm mapping to intron 1 and the right arm mapping to intron 14. The absence of protein expression was confirmed by western blot analysis on mouse embryonic fibroblasts, brain, and liver extracts. (J:177760)