这个SIRT2-Tg flox-STOP-flox构建是为了在5'到3'的位置插入FRT序列、CMV-IE增强子/鸡β-actin/兔β-globin融合启动子(CAG,来自pCAGGS载体)、一个含loxP标签的转录终止子、小鼠Sirt2基因的cDNA序列,以及一个介于PGK和hygro之间的polyA-PGK-hygro序列(其中PGK和hygro之间还有一个FRT位点),所有这些元件都被插入到小鼠胶原蛋白A1(Col1a1)基因的3'非编码区。(来源:J:177468)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
(C57BL/6 x 129S4/SvJae)F1
Targeted
插入
--
1
24
4

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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