使用靶向载体,替换了目标基因的2.6kb编码区域,包括所有6个外显子,用FRT引导的PGK-抗氨苄西林抗性 cassette。通过Flp介导的重组移除了neo cassette。通过定量RT-PCR在盲肠提取物中确认了转录表达的缺失。(来源:J:177506, J:177550)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
129
Targeted
插入,基因内删除
--
1
--
7

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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