The short hairpin RNA (shRNA) against Pion (5'-TCCCGGAACTCCATGATTGACAAATTTCAAGAGAATTTGTCAATCATGGAGTTCCTTTTTA-3') was inserted into the locus via RMCE with Gt(ROSA)26Sortm1(CAG-flpe)Arte. The shRNA sequence is under the regulation of the tetO operator and a codon optimized tet repressor (itetR) under the control of a CAG promoter. (J:163997)