Lgr5^{tm2(Hbegf/EGFP)Fjs}

A targeting vector was generated so that a DTR-EGFP-pA-loxP-neo-loxP cassette (diphtheria toxin (DT) cDNA linked in-frame to EGFP polyA-floxed neo) was inserted immediately after the ATG codon in exon 1 of the Lgr5 locus (deleting the remainder of exon 1 and splice donor site in intron 1 - a 212 bp deletion) by homologous recombination in C2 ES cells. Correctly targeted clones were transfected with an cre plasmid to remove the neo cassette. (J:177145)