通过Cre介导的切除,去除了2到5号外显子以及neo cassette,蛋白表达缺失通过脑组织的 Western blot 分析得到了确认(来源:J:176956)
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Cre mediated excision removed exons 2 through 5 and the neo cassette. The absence of protein expression was confirmed by western blot analysis on brain extracts. (J:176956)