大部分exon 1被一个floxed neo/kan cassette替换,通过E9.5胚胎提取物的 Western blot分析证实了没有蛋白表达。(来源:J:176798)
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Most of exon 1 was replaced with a floxed neo/kan cassette. The absence of protein expression was confirmed by western blot analysis on E9.5 embryo extracts. (J:176798)