通过同源重组技术,将一个Frt-flankedneo cassette,随后跟着一个loxP位点插入Fstl1基因的第2外显子后面,然后在第2外显子之前插入另一个loxP位点。杂交产生了雄性转基因小鼠,这些小鼠与FVB雌性杂交,产生后代并用Flpe剔除工具去除neo cassette,接着与CMV-cre转基因动物杂交,以删除第2外显子。(来源:J:176532)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
(C57BL/6 x 129S4/SvJae)F1
Targeted
插入,基因内删除
--
1
--
1

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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