A construct containing a short hairpin RNA targeting Mir145 driven by the H1/tetO operator fusion promoter followed by a CAG promoter driving expression of the tetR regulatory cassette was inserted into the locus via flp-mediated recombination using RMCE with Gt(ROSA)26Sortm1(CAG-flpe)Arte. Doxycycline-treatment is required to activate expression of the shRNA. (J:176266)