从3号外显子到6号外显子被替换了新插入的cassette。通过在小鼠胚胎成纤维细胞提取物中进行 Western blot 分析,证实了没有蛋白质表达(来源:J:174947)
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Exons 3 through 6 were replaced with a neo cassette. The absence of protein expression was confirmed by western blot analysis on mouse embryonic fibroblast extracts. (J:174947)