通过Cre介导的重组,去除了exon 2和neo cassette,蛋白表达缺失通过脑、睾丸和肺组织的 Western blot 分析得到了确认(来源:175247)
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Cre-mediated recombination removed exon 2 and the neo cassette. The absence of protein expression was confirmed by western blot analysis on brain, testis, and lung extracts. (J:175247)