整个编码区域被替换为转基因小鼠Thrb2的内切互补DNA(cDNA)和一个敲除neo cassette,通过同源重组实现。通过Cre介导的重组,neo cassette被剔除了。 Western blot结果显示,E17.5时,小鼠中Thrb2蛋白持续表达,而Nrl不表达。基因定位分析显示,P7时,同源纯合和杂合个体视网膜外神经源性层存在Thrb mRNA的异位表达。(来源:174592)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
C57BL/6
Targeted
插入,基因内删除
--
1
5
1

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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