在内皮1号 intron插入的基因陷阱 cassette,大多数组织中mRNA水平减少了大约100倍。在纯合小鼠的脑部, Western blot分析并未检测到蛋白质。 (来源:基因陷阱 cassette 被插入到内皮1号 intron,导致大部分组织的mRNA显著减少,且在纯合小鼠的脑中,通过 Western blot 实验无法检测到相应蛋白质。)
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The gene trap cassette inserted in intron 1. mRNA levels were decreased by over 100-fold in most tissues. Western blot analysis failed to detect protein in brains from homozygous mice.