从4到6号外显子被替换成带有IRES-lacZ报告基因和neo cassette。通过在肝脏提取物中进行 Western blot 分析,确认了没有蛋白质表达(来源:J:174999)
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Exons 4 through 6 were replaced with an IRES-lacZ reporter and neo cassette. The absence of protein expression was confirmed by western blot analysis on liver extracts. (J:174999)