通过将CAG启动子与3.5kb的lacZ基因通过一个由loxP标记的终止信号分隔,构建出了一个表达载体。lacZ序列被连接到了一个核定位信号。经过8.0kb的线性化片段被注入BDF1的卵子中,产生了转基因小鼠。产生了3个杂交瘤品系(编号1,2,3),每个品系含有的转基因拷贝数分别为5, 10, 和15个。(来源:J:98285)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Gene Expression
Related Disease
Reference
(C57BL/6 x DBA/2)F1
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1

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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