Ccdc39^prh

Identified in an ENU screen and mapped to Chromosome 3 0-76 (4 Mb). Genotyping of affected mice (n=51) indicated complete concordance between homozygosity for a chr3:g.33731448A>T variant and the hydrocephalus phenotype. Mutants expressed two atypical transcripts but no wild-type transcript, a result of a failure in splicing intron 7 and cryptic alternative splicing in exon 7. Immunoblotting of wild-type and mutant brain lysates indicated no protein is expressed from this allele in the mutant mice. (J:174027, J:253968)