在exon 2上游插入了loxP位点,然后在exon 5下游插入了loxP标记的诺卡因和胸腺嘧啶激酶选择性载体。通过Cre介导的重组,去除了诺卡因载体。随后,通过与携带Hprttm1(CMV-cre)Brd转基因小鼠杂交,从靶向正确的小鼠中删除了exons 2到5。(来源:J:94077)
Legend:
cx: complex: > 1 genome feature ot: other: hemizygous, indeterminate,... (F): Female
(M): Male
N: normal phenotype
(#): related diseases count