在exon 1的上游插入了一个loxP位点,然后在exon 2下游紧接一个由FRT引导的PGKneo cassette的loxP座。之后,通过与携带Tg(ACTFLPe)9205Dym转基因小鼠杂交,从定向靶向的鼠中去除了选择座。(来源:J:94077)
Legend:
cx: complex: > 1 genome feature ot: other: hemizygous, indeterminate,... (F): Female
(M): Male
N: normal phenotype
(#): related diseases count