Tg(CMV-CASP3)17Edge

The Mos-iCsp3 transgene was designed with (from 5' to 3') a CMV promoter, a loxH site, a beta-galactosidase (lacZ) gene, a V5-tag (derived from the paramyxovirus of simian virus 5 (SV5)), a 6x-histidine-tag, a polyadenylation (polyA) signal, and a loxP . This floxed-lacZ-STOP cassette was followed by a myristoylation sequence, two tandem FK506-binding sites (Fvs), a human Caspase 3 (CASP3) gene, a haemagglutinin (HA) tag, and a second (polyA) signal. Lines 14 and 17 were generated. (J:173515)