在exon 1上游插入了loxP位点,然后在exon 1下游插入了带有floxed neo cassette的片段。通过Cre介导的重组,去除了exon 1和neo cassette。通过E9.5胚胎提取物的RT-PCR,确认了转录表达的缺失。(来源:J:171997)
Legend:
cx: complex: > 1 genome feature ot: other: hemizygous, indeterminate,... (F): Female
(M): Male
N: normal phenotype
(#): related diseases count