7号和8号外显子被替换成lacZ和neo插入片段。通过在心脏、肌肉、脾脏、肾脏和肝脏的提取物中进行 Western blot 分析,确认了没有蛋白质表达(来源:J:173192)
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Exons 7 and 8 were replaced with a lacZ and neo cassette. The absence of protein expression was confirmed by western blot analysis on heart, muscle, spleen, kidney, and liver extracts. (J:173192)