这个靶向构建设计用于替换29-34号外显子(编码细胞内域),接着插入CreERT2序列、核定位信号,然后是C端的6xMyc标签、2个SV40polyA序列,以及由Frt-flanked Pgk1/EM7启动子驱动的诺卡因选择标记。Cre-ERT2融合基因是将Cre重组酶与人类雌激素受体配体结合域的G400V/M543A/L544A三重突变融合而成的。(来源:J:172930)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
C57BL/6NTac-Tg(HBB-lacZ)ALey/Ley
Targeted
插入,基因内删除
--
1
7
6

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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