编码区域被替换为EGFP、IRES和一个puro序列。通过ES细胞提取物的 Western blot 分析,证实了没有蛋白质表达(来源:J:172565)。
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The coding region was replaced with EGFP, IRES, and a puro cassette. The absence of protein expression was confirmed by western blot analysis on ES cell extracts. (J:172565)