Prap1基因由5个外显子组成,1至5号编码区域通过同源重组方法进行了靶向(NCBI accession NM_009475.1)。通过Southern杂交分析确认了目标基因的失活。使用新制的PRAP1兔抗血清进行的 Western blot结果显示,纯合突变小鼠的肠和子宫整体组织中未检测到蛋白表达。免疫荧光染色进一步证实了十二指肠组织中无可检测到的信号。(文献引用:J:171883, J:304152)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
129S5/SvEvBrd
Targeted
基因内删除
--
1
--
2

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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