单个编码外显子通过同源重组被贝塔伽马eo/puro载体替换。qRT-PCR和 Western blotting 验证了雄性单显性突变小鼠中表达缺失。(来源:J:171883, J:237065)
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The single coding exon was replaced by a betageo/puro cassette by homologous recombination. qRT-PCR and Western blotting confirmed the absence of expression in male hemizygous mutant mice. (J:171883, J:237065)