在肝脏和白色脂肪组织的提取物中,通过 Western blot 分析确认了编码区域的 904 个碱基被替换为带有 IRES-lacZ 报告基因和 neo cassette 的情况,没有检测到蛋白质表达。(来源:J:171942)
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904 bp of the coding region was replaced with an IRES-lacZ reporter and neo cassette. The absence of protein expression was confirmed by western blot analysis on liver and white adipose tissue extracts. (J:171942)