通过Cre介导的重组,去除了插入在内皮4号染色体上的含诺美西林抗性基因元件的loxP位点。 Western blot结果显示,对多柔比星处理的胚胎成纤维细胞中,恢复了蛋白质表达。(来源:J:171821)
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Cre-mediated recombination removed the loxP site flanked neomycin resistance gene cassette that was inserted into intron 4. Western blot analysis confirmed restored protein expression in doxorubicin-treated mouse embryonic fibroblasts. (J:171821)