Chat/Slc18a3^tm1Vpra

One loxP sequence was added 260 bp upstream from the translational initiation codon of Slc18a3 (within the first intron of Chat), and a second loxP was added approximately 1.5 kb downstream from the stop codon. This flanking region included the second exon of Chat. The TK-Neo cassette was added immediately after the second loxP and was followed by a third loxP (all in the same direction). QPCR confirmed reduced transcript expression in the brain and spinal cord. (J:153908)