Exon 2被标记为可删除。通过Cre介导的重组,去除了Exon 2。蛋白表达缺失通过在E15.5时对整个胚胎和肺组织的 Western blot 分析得到了确认(来源:J:171347)
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Exon 2 was floxed. Cre-mediated recombination removed exon 2. The absence of protein expression was confirmed by western blot analysis on E15.5 whole embryo and lung extracts. (J:171347)