通过Cre介导的重组,移除了27号外显子(包含EGFP和neo cassette)。蛋白表达缺失通过睾丸提取物的 Western blot 实验得到了确认(来源:J:171225)
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Cre-mediated recombination removed exon 27, the EGFP, and the neo cassette. The absence of protein expression was confirmed by western blot analysis on testes extracts. (J:171225)