在exon 10的上游插入了一个loxP位点。在exon 10下游插入了一个FRT-介导的neo cassette,它带有5'的loxP位点。通过Flp介导的重组移除了neo cassette。随后,Cre介导的重组去除了exon 10,导致短剪接变异体的敲除。通过 Western blot 分析确认了短形式的缺失以及长形式的表达。(来源:J:170494)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
(129X1/SvJ x 129S1/Sv)F1-Kitl+
Targeted
插入,基因内删除
--
1
11
3

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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