开放阅读框被替换了为一个neo cassette。通过在杂合子大脑提取物中的 Western blot 分析,确认了蛋白质表达量减少了50%。(来源:J:171182)
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The open reading frame was replaced by a neo cassette. A 50% reduction in protein expression was confirmed by western blot analysis on heterozygous brain extracts. (J:171182)