在exon 4上游插入了loxP位点,然后在exon 6下游插入了带有floxed neo cassette的片段。对后肢和心脏的提取物进行 Western blot 分析后,结果显示蛋白表达显著减少。这个基因型表现为部分缺失(J:170869)。
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A loxP site was inserted upstream of exon 4. A floxed neo cassette was inserted downstream of exon 6. Western blot analysis on hindlimb and cardiac extracts confirmed largely absent protein expression. This allele is hypomorphic. (J:170869)