用于同源重组的靶向载体通过BAC重组技术构建。载体包含了tamoxifen诱导的Cre(cre/ERT2)序列、SV40polyA信号以及Frt引导的PGK-neo cassette,它们被定向插入Lrig1的内源性ATG位点。随后,这些转基因动物与表达Flpe的个体杂交,以从生殖细胞中去除neo基因。(来源:J:186084)
Legend:
cx: complex: > 1 genome feature ot: other: hemizygous, indeterminate,... (F): Female
(M): Male
N: normal phenotype
(#): related diseases count