无内含子的基因被整体删除,并用PolII neo pA表达载体替换。通过Kdo2-Lipid A诱导后,通过肺和肝脏提取物的 Western blot 分析确认了蛋白表达的缺失。(来源:J:170807)
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The intron-less gene was deleted in its entirety and replaced by the PolII neo pA expression cassette. The absence of protein expression was confirmed by western blot analysis on lung and liver extracts after Kdo2-Lipid A induction. (J:170807)