Myt1^{tm1(EGFP/icre)Ldh}

A targeting vector was designed to disrupt exon 2 with a fusion of EGFP and the modified Cre-recombinase, iCre , followed by a loxP site, and an frt-flanked neomycin (neo) resistance cassette. The vector also changed the initiation codon at the beginning of exon 2 to a GTG to ensure translation of the EGFP/iCre fusion protein. The neomycin cassette was removed by crossing mice with a transgenic strain expressing Tg(ACTFLPe)9205Dym leaving a single frt site and a loxP site downstream of the EGFP/iCre fusion. Northern blot confirmed the absence of mRNA. At E18, fluorescent cells are distributed throughout the spinal cord. (J:169913)