在exon 2中插入了lacZ和floxed neo cassette,随后通过Cre介导的重组移除了neo cassette。通过脑组织提取物的 Western blot 分析,确认了蛋白表达的缺失。(来源:J:125317)
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A lacZ and floxed neo cassette were inserted into exon 2. Cre-mediated recombination removed the neo cassette. The absence of protein expression was confirmed by western blot analysis on brain extracts. (J:125317)