5'剪接底物EGFP、poly(A)序列和含floxed neo cassette的替换了exon 1和intron 1的编码区域。通过Cre介导的重组,neo cassette被去除了。通过RT-PCR确认了转录量减少了10倍。(来源:J:169456)
Legend:
cx: complex: > 1 genome feature ot: other: hemizygous, indeterminate,... (F): Female
(M): Male
N: normal phenotype
(#): related diseases count